Save your seat for our upcoming webinar: Enzymatic solutions for overcoming the challenges of FFPE DNA in NGS library prep

Sign up for our upcoming webinar. Unable to view this email? [View online]( Dear {NAME}, Please join us for our upcoming webinar highlighting Enzymatic solutions for overcoming the challenges of FFPE DNA in NGS library prep. [Register Today!]( Enzymatic solutions for overcoming the challenges of FFPE DNA in NGS library prep Tuesday, February 27th 1:00 PM EST In cancer genomics, a common source of DNA is formalin-fixed, paraffin-embedded (FFPE) tissue from patient surgical samples, where in most cases high quality fresh or frozen tissue samples are not available. FFPE DNA poses many notable challenges for preparing NGS libraries, including low input amounts and highly variable damage from fixation, storage, and extraction methods. We developed a new DNA damage repair enzyme mix, enzymatic fragmentation mix, and library amplification PCR master mix, optimizing the activities of these mixes using FFPE DNA samples of varied quality to maximize yield, WGS library quality, and target enrichment library performance. This new suite of enzyme mixes allows even highly damaged FFPE samples to achieve high-quality libraries with sufficient input for hybrid capture. Increasing the useable reads and coverage enables robust detection of somatic variants as demonstrated using both reference standard DNA and patient-derived FFPE samples. The use of enzymatic fragmentation and a flexible PCR master mix make this FFPE library prep workflow compatible with high-throughput and automation-based workflows. Maggie Heider Development Associate Maggie Heider received her B.A. in Biology from the College of the Holy Cross and her Ph.D. from the University of Massachusetts Medical School. Her graduate work focused on characterizing the protein complexes that regulate intracellular membrane trafficking and developing novel tools and assays to study their activity. Upon joining NEB in 2016, she switched her focus to enzymology and has spent the last several years developing NGS library preparation reagents and workflows including DNA library prep kits, enzymatic DNA repair, enzymatic fragmentation, PCR reagents, and multiplex oligos. Her recent focus is on developing reagents and workflows for clinical sample types, particularly FFPE DNA samples. --------------------------------------------------------------- Wishing you success in your research, New England Biolabs, Inc. Telephone: (978) 927-5054 Toll Free: (U.S. Orders) 1-800-632-5227 Toll Free: (U.S. Tech) 1-800-632-7799 info@neb.com --------------------------------------------------------------- New England Biolabs is a [Certified B Corporation®](. --------------------------------------------------------------- [New Products]( [Special Offers]( [Technical Support]( [Customer Feedback]( --------------------------------------------------------------- Products and content are covered by one or more patents, [trademarks]( and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of these products may require you to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com. Your purchase, acceptance, and/or payment of and for NEB's products is pursuant to NEB's [terms of sale](. NEB does not agree to and is not bound by any other terms or conditions, unless those terms and conditions have been expressly agreed to in writing by a duly authorized officer of NEB. B Corporation® is a registered trademark of B Lab IP, LLC, Inc. © Copyright 2024, New England Biolabs, Inc.; All rights reserved. New England Biolabs, Inc. is an ISO 9001, ISO 14001 and ISO 13485 certified facility. NEB does not intend to inundate you with email messages. If you would like to update your email preferences or unsubscribe from the list, please [click here](. New England Biolabs, Inc. | 240 County Road | Ipswich, MA 01938-2723