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Dear {NAME},
Please join us for our upcoming two part webinar series highlighting our new [NEBNext UltraExpressâ¢]( products for DNA & RNA library prep. [Register Today!]( Streamlined for Speed: NEBNext UltraExpress⢠for RNA library prep Tuesday, January 30th 1:00 PM EST RNA sequencing (RNA-seq) has become an invaluable tool in the study of biology, but it often requires multiple days to process samples from total RNA to final libraries. When working with samples in a high-throughput capacity in particular, protocol transfer to automation and the time required to prepare libraries can be a great hindrance to processing samples efficiently. Weâve developed a new protocol that allows users to easily generate robust RNA-seq libraries from a variety of sample types in a single day from 25-250 ng total RNA. Compatible with poly(A) enrichment or ribosomal RNA (rRNA) depletion, this new workflow has reduced preparation time by about 40% compared to leading library preparation kits, without sacrificing quality of libraries or sequencing metrics. In addition to faster preparation times, we introduced flexibility for users with a protocol that is automation friendly and allows users to implement a single protocol for all RNA inputs within the kit range. In addition to master mixed components and reduced incubation times, the reduction in bead cleanup steps significantly reduces hands-on time for library preparation, simplifying and streamlining the process. Furthermore, all steps in the workflow have been optimized to make them automation friendly.During this webinar, we will describe how the NEBNext UltraExpress⢠RNA library preparation workflow provides a great advantage in efficiently generating strand specific/directional RNA-seq libraries while simultaneously maintaining high quality sequencing results, including 5â-3â transcript coverage, excellent transcript abundance detection, and high correlation in transcript expression between replicates and across inputs. We expect these library preparation methods will serve in a myriad of NGS applications, including clinical and diagnostic settings. Chen Song Development Scientist
Chen is a development scientist at NEB focusing on developing NGS workflows for DNA and RNA sequencing and is passionate about its applications in molecular diagnosis of cancer, infectious disease, and autoimmune disease. Prior to joining NEB, Chen had 10 years of research experience in assay development for biomarker discovery and therapeutics. During the past 8 years, Chen has contributed to the development of several key NEBNext® products, including the Immune-Seq kit, Multiplex Oligos (UDI Adaptors and Primers) for Illumina, and most recently, the NEBNext UltraExpress DNA Library Prep Kit. Her professional expertise and interests include molecular diagnostics technologies, biomarker discovery and applications, and data analysis and visualization.
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