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Looking for solutions for cell-free protein expression & purification?

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neb.com

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NEBUpdates@neb.com

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Wed, Oct 25, 2023 07:04 PM

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The NEBExpress Cell-free E. coli Protein Synthesis System can generate high yields of proteins. OCTO

The NEBExpress Cell-free E. coli Protein Synthesis System can generate high yields of proteins. OCTOBER 2023 Unable to view this email? [View online]( Looking for solutions for cell-free protein expression & purification? --------------------------------------------------------------- Featured technical note: A high performance E. coli cell lysate-based system for in vitro protein synthesis Cell-free protein synthesis (CFPS) systems based on bacterial cell lysates have been widely used for an array of applications. These systems offer several advantages; for example, the tight coupling of translation and transcription from prokaryotic cells is preserved in the lysate, rendering the protein synthesis process exceptionally efficient. The NEBExpress® Cell-free E. coli Protein Synthesis system was developed using several strategies to enhance performance, ease of use, and ensure robustness. These include the use of an E. coli strain genetically engineered to maximize the stability of template DNA and RNA and the protein products, a highly optimized reaction buffer, and a stringent biomanufacturing process. In this technical note, we examine how the NEBExpress Cell-free E. coli Protein Synthesis System performs in several frequently encountered applications, and demonstrate this system’s high performance and versatility. The NEBExpress Cell-free E. coli Protein Synthesis System can generate high yields of proteins from a wide range of targets, and can be used to optimize synthesis temperature. [Download Technical Note]( [Learn More]( --------------------------------------------------------------- Featured products: Purify tagged proteins with our magnetic beads, columns, and resins [Nickel spin columns](, [magnetic beads]( and [resin]( enable rapid purification of His-tagged proteins [Amylose resins]( for purification of MBP-tagged proteins are available in a variety formats ([standard](, [high flow]( and [magnetic]() [Chitin resin]( allows for rapid purification of CBD-tagged proteins Remove affinity tags following your purification with [TEV Protease]( --------------------------------------------------------------- Explore the full [protein expression & purification portfolio]( --------------------------------------------------------------- New to protein purification? Download our brochure for [Purification Beads, Columns, and Resins]( --------------------------------------------------------------- New England Biolabs is a [Certified B Corporation®](. --------------------------------------------------------------- [New Products]( [Special Offers]( [Technical Support]( [Customer Feedback]( --------------------------------------------------------------- Products and content are covered by one or more patents, [trademarks]( and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of these products may require you to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com. Your purchase, acceptance, and/or payment of and for NEB's products is pursuant to NEB's [terms of sale](. NEB does not agree to and is not bound by any other terms or conditions, unless those terms and conditions have been expressly agreed to in writing by a duly authorized officer of NEB. B Corporation® is a registered trademark of B Lab IP, LLC, Inc. © Copyright 2023, New England Biolabs, Inc.; All rights reserved. New England Biolabs is an ISO 9001, ISO 14001 and ISO 13485 certified facility. NEB does not intend to inundate you with email messages. If you would like to update your email preferences or unsubscribe from the list, please [click here](. New England Biolabs, Inc. | 240 County Road | Ipswich, MA 01938-2723

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