The NEBExpress Cell-free E. coli Protein Synthesis System can generate high yields of proteins. OCTOBER 2023
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A high performance E. coli cell lysate-based system for in vitro protein synthesis Cell-free protein synthesis (CFPS) systems based on bacterial cell lysates have been widely used for an array of applications. These systems offer several advantages; for example, the tight coupling of translation and transcription from prokaryotic cells is preserved in the lysate, rendering the protein synthesis process exceptionally efficient. The NEBExpress® Cell-free E. coli Protein Synthesis system was developed using several strategies to enhance performance, ease of use, and ensure robustness. These include the use of an E. coli strain genetically engineered to maximize the stability of template DNA and RNA and the protein products, a highly optimized reaction buffer, and a stringent biomanufacturing process. In this technical note, we examine how the NEBExpress Cell-free E. coli Protein Synthesis System performs in several frequently encountered applications, and demonstrate this systemâs high performance and versatility. The NEBExpress Cell-free E. coli Protein Synthesis System can generate high yields of proteins from a wide range of targets, and can be used to optimize synthesis temperature. [Download Technical Note]( [Learn More](
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