Golden Gate Assembly? Think NEBridge.

Learn more about our products for Golden Gate assembly. JULY 2023 Unable to view this email? [View online]( Assemble With Confidence using NEBridge® Golden Gate Assembly NEBridge Golden Gate Assembly reagents and NEBridge™ Ligase Fidelity Web Tools power high-complexity and high-fidelity Golden Gate Assemblies and are enabling advancements in synthetic biology research. [Learn More]( - Clone seamlessly, with no scars remaining after assembly - Perform single insert cloning in just 5 minutes using our fast protocols - Generate libraries with high efficiencies - Assemble multiple fragments (2–50+) in order, in a single reaction - Experience high efficiency, even with regions of high GC content and areas of repeats - Use with a broad range of fragment sizes (<100 bp to >15 kb) - Simplify reaction setup with our suite of primer design and ligase fidelity tools For convenience and value, NEB provides two optimized kits containing Type IIS restriction enzymes and a cloning vector: [NEBridge Golden Gate Assembly Kit (BsmBI-v2)]( [NEBridge Golden Gate Assembly Kit (BsaI-HF® v2)]( For added flexibility, use NEBridge Ligase Master Mix with your choice of NEB Type IIS restriction enzymes: [NEBridge Ligase Master Mix]( --------------------------------------------------------------- Try our suite of free online NEBridge™ tools These tools enable the design of complex, high-fidelity Golden Gate Assemblies: [NEBridge Golden Gate Assembly Tool]( Design assemblies and primers with appropriate Type IIS restriction sites and overhangs [NEBridge Ligase Fidelity Viewer™]( Visualize overhang ligation preferences [NEBridge GetSet™]( Predict high-fidelity overhang sets [NEBridge SplitSet™]( Split DNA sequence for scarless high-fidelity assembly --------------------------------------------------------------- [Did you catch our latest podcast?]( NEB Senior Tech Support Scientist Rachel Carver-Brown explains site-directed mutagenesis and multi-site mutagenesis techniques. --------------------------------------------------------------- We want you to help us create our 2024 calendar! We are looking for nature-themed photography for inclusion in our 7th annual NEB® Passion in Science Calendar. Please submit your work for consideration, by supplying a high-resolution image, along with your contact information, by September 15th.* If your work is selected for inclusion in the calendar, you will, of course, be credited in the calendar and celebrated online and on social media. You will also receive the following: - $500 in NEB product credit or $500 to donate to the charity of your choice - 10 copies of the calendar featuring your work to hand out to your nearest and dearest - A personal reminder of the beauty of reflection [Submit Your Photo Today]( --------------------------------------------------------------- Product update: NEB enzyme formulations and buffers – now with Recombinant Albumin (rAlbumin) To address the increased need for BSA-free reagents, NEB has switched our BSA-containing reaction buffers to [rAlbumin-containing buffers](. We are also in the process of transitioning our enzyme formulations to contain [rAlbumin](. NEB has rigorously tested these changes and has not seen a difference in performance. rAlbumin is now supplied with 120 enzymes, including our popular restriction enzymes, [BsaI-HF®v2]( [BamHI-HF]( and [NotI-HF](. Enzymes that have transitioned in July include: - [PvuII]( - [DpnI]( - [PflMI]( - [BcoDI]( - [SgrAI]( - [HpyCH4III]( - [HpyCH4V]( [Learn More]( New England Biolabs is a [Certified B Corporation®](. --------------------------------------------------------------- [New Products]( [Special Offers]( [Technical Support]( [Customer Feedback]( --------------------------------------------------------------- Products and content are covered by one or more patents, [trademarks]( and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of these products may require you to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com. Your purchase, acceptance, and/or payment of and for NEB's products is pursuant to NEB's [terms of sale](. NEB does not agree to and is not bound by any other terms or conditions, unless those terms and conditions have been expressly agreed to in writing by a duly authorized officer of NEB. B Corporation® is a registered trademark of B Lab IP, LLC, Inc. © Copyright 2023, New England Biolabs, Inc.; All rights reserved. New England Biolabs, Inc. is an ISO 9001, ISO 14001 and ISO 13485 certified facility. NEB does not intend to inundate you with email messages. 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