Streamline your rolling circle amplification with our new kit, learn about our featured enzyme for innovation, and get updated on BSA-free enzymes. APRIL 2023
Unable to view this email? [View online]( New product:
phi29-XT RCA Kit
This new kit includes everything you need for rolling circle amplification (RCA) and features phi29-XT DNA Polymerase, an engineered polymerase with improved thermostability and sensitivity. It is capable of generating high yields in less time.
- Amplify from as little as 1 fg of circular DNA input
- Use with a variety of input materials
- Enable multiple downstream applications without further processing steps
- Kit includes dNTPs and exo-resistant random primers Overview of the phi29-XT RCA Kit [Product Information]( [View Webinar On Demand]( Have you heard of Enzymes for Innovation? The NEB catalog highlights a wide variety of enzyme functionalities found in nature or engineered for specific purposes. However, in molecular biology, new tools can often lead to new discoveries. Taking advantage of the enzymology expertise at NEB, we are offering novel enzymes with interesting and unique activities for manipulating DNA, RNA, proteins and glycans, even if specific applications for them have yet to be discovered. Our hope is that by engaging researchers' imaginations, our âEnzymes for Innovationâ initiative will enable the development of new molecular techniques that so often lead to new discoveries. Featured Enzyme for Innovation Immobilized T4 DNA Ligase is a slurry of magnetic beads coated with T4 DNA Ligase to produce a 10 mg/ml solution (50% glycerol) with an effective concentration of 60 cohesive end units (CEU) per microliter of slurry. Following a reaction, the enzyme can be removed using a magnet and can be re-used. [Immobilized T4 DNA Ligase]( [Learn More About
Enzymes for Innovation](
Looking for an enzyme functionality that is not currently available? Contact us at enzymesforinnovation@neb.com.
--------------------------------------------------------------- Product update:
NEB enzyme formulations and buffers â
now with Recombinant Albumin (rAlbumin) To address the increased need for BSA-free reagents, NEB has switched our BSA-containing reaction buffers to [rAlbumin-containing buffers](. We are also in the process of transitioning our enzyme formulations to contain [rAlbumin](. NEB has rigorously tested these changes and has not seen a difference in performance. rAlbumin is now supplied with 103 enzymes, including our popular restriction enzymes, [BsaI-HF®v2]( [BamHI-HF]( and [NotI-HF](. Enzymes that have transitioned in April include:
- [ApeKI](
- [BamHI](
- [BseRI](
- [BstUI](
- [DraI](
- [EciI](
- [MslI](
- [NgoMIV](
- [Sau96I]( [Learn More](
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