Our new high-fidelity Cas9 reduces off-target cleavage, details on our Molecular Biology workshop, and more!

Find the latest new products and product updates, as well as details on our annual Molecular Biology Workshop. MARCH 2023 Unable to view this email? [View online]( Now available: EnGen® Spy Cas9 HF1 for Reduction of Off-Target Effects in Genome Editing Workflows Reduction of off-target effects, the unintended cleavage and mutations at untargeted genomic loci with similar homology to the target, can be achieved by layering strategies such as guide selection and nuclease engineering. [EnGen Spy Cas9 HF1]( is an engineered Spy Cas9 that reduces off-target effects while maintaining on-target editing efficiency. Spy Cas9 HF1 demonstrates increased sensitivity to mismatches by showing the greatest ratio of on-target cleavage to average cleavage of off-targets in vitro Comparison of the in vitro tolerance of mismatches between the guide RNA sequence and target DNA sequence of EnGen Spy Cas9 NLS, EnGen Spy Cas9 HF1, and other commercially available high fidelity Cas9 variants. The percent substrate cleavage for each RNP complex was measured by capillary electrophoresis. Results were graphed as a heat map with white representing no cleavage and increasing intensity of blue indicating increasing percent cleavage. [Product Information]( Interested in learning molecular biology in 2 weeks? Register for our Molecular Biology Summer Workshop This two-week long course covers DNA cloning, gene expression analysis, DNA sequencing (including NextGen and RNA-seq), genomics, bioinformatics, CRISPR/Cas9, RNA interference, PCR and quantitative PCR, protein expression systems and proteomics in-depth. Learn hands-on techniques used in gene expression analysis including microarray analysis, RNAi and quantitative RT-PCR, bioinformatics and genomics and proteomics. No previous experience in molecular biology is required or expected. Where: Smith College, Northampton, MA When: July 9th – 22nd, 2023 [Register Today]( --------------------------------------------------------------- Product update: NEB enzyme formulations and buffers – now with Recombinant Albumin (rAlbumin) To address the increased need for BSA-free reagents, NEB has switched our BSA-containing reaction buffers to [rAlbumin-containing buffers](. We are also in the process of transitioning our enzyme formulations to contain [rAlbumin](. NEB has rigorously tested these changes and has not seen a difference in performance with these changes. To date, 97 enzymes are now supplied with rAlbumin, including our popular restriction enzymes, [BsaI-HF®v2]( [BamHI-HF]( and [NotI-HF](. Enzymes that have transitioned in March include: - [AfeI]( - [EarI]( - [HinFI]( - [SacII]( - [XmaI]( [Learn More]( --------------------------------------------------------------- New England Biolabs is a [Certified B Corporation®](. --------------------------------------------------------------- [New Products]( [Special Offers]( [Technical Support]( [Customer Feedback]( --------------------------------------------------------------- Products and content are covered by one or more patents, [trademarks]( and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of these products may require you to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com. Your purchase, acceptance, and/or payment of and for NEB's products is pursuant to NEB's [terms of sale](. NEB does not agree to and is not bound by any other terms or conditions, unless those terms and conditions have been expressly agreed to in writing by a duly authorized officer of NEB. IDT® and Alt-R® are registered trademarks of Integrated DNA Technologies, Inc. SIGMA-ALDRICH® is a registered trademark of Merck KGAA. GenScript® is a registered trademark of Nanjing GenScript Biotech Co., LTD. GenCRISPR™ is a trademark of GenScript USA Inc. B Corporation® is a registered trademark of B Lab IP, LLC, Inc. © Copyright 2023, New England Biolabs, Inc.; All rights reserved. New England Biolabs, Inc. is an ISO 9001, ISO 14001 and ISO 13485 certified facility. NEB does not intend to inundate you with email messages. If you would like to update your email preferences or unsubscribe from the list, please [click here](. New England Biolabs, Inc. | 240 County Road | Ipswich, MA 01938-2723