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Find out how NEB can support your infectious disease research, and much more

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neb.com

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NEBupdates@neb.com

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Fri, Apr 29, 2022 07:36 PM

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Featured products for RT-qPCR, DNA assembly, DNA and RNA nucleic acid purification. APRIL 2022 Unabl

Featured products for RT-qPCR, DNA assembly, DNA and RNA nucleic acid purification. APRIL 2022 Unable to view this email? [View online]( It’s time to think differently. Many of you know NEB® as a trusted reagent supplier to the life science community, but did you know that we also offer a portfolio of products that can be used in infectious disease research, development of diagnostics and therapies, as well as in epidemiological studies and disease surveillance? [Many of our products have played a role in the global response to SARS-CoV-2]( from supporting the development of COVID-19 diagnostics and vaccines to [improving the speed and accuracy of Illumina® and Nanopore-based COVID-19 sample analysis](. They can also be utilized with other infectious diseases, such as influenza and malaria. Find out how NEB can support your infectious disease research and development. [Learn More]( --------------------------------------------------------------- Featured product: Luna® Probe One-Step RT-qPCR 4X Mix with UDG (No ROX) Ideal for instruments that do not require the ROX passive reference dye, the Luna Probe One-Step RT-qPCR 4X Mix with UDG (No ROX) enables sensitive detection of target RNA sequences, with robust performance in multiplex applications of up to 5 targets. Detection of RNA viruses using the Luna Probe One-Step RT-qPCR 4X Mix with UDG (No ROX) RT-qPCR targeting SARS-CoV-2 (N1 target), Zika (POLY target), Dengue (E target), Chikungunya (E2 glycoprotein target) was performed using the Luna Probe One-Step RT-qPCR Mix with UDG (No ROX). Performance in 20 μl reactions was evaluated on a Bio-Rad® CFX96 real-time instrument over a 5-log range of template (100,000–10 copies/reaction). Templates including Synthetic SARS-CoV-2 RNA Control 2 (Twist Biosciences, SKU: 102024), Synthetic Zika virus (ZIKV) RNA (ATCC® VR-3252SD™), Synthetic RNA from Dengue virus type 2 (ATCC VR-3229SD™), Synthetic RNA from Chikungunya virus (ATCC VR-3246SD™), were all individually diluted in 10 ng of Jurkat total RNA (BioChain, #R1255815-50). Sensitive, linear performance can be observed in the amplification of all viral targets. [Product Information]( Featured product: NEBuilder® HiFi DNA Assembly Assembling DNA fragments is a key part of both synthetic biology techniques and cloning. [NEBuilder HiFi DNA Assembly]( enables virtually error-free joining of DNA fragments, even those with 5´- and 3´-end mismatches. This flexible kit enables simple and fast seamless cloning utilizing a proprietary high-fidelity polymerase, and offers improved performance over other commercially-available kits. Featured protocols: [Protocol for assembling annealed DNA oligonucleotides and a double-stranded DNA vector using NEBuilder HiFi DNA Assembly]( [Protocol for Bridging double-stranded DNA with a single-stranded DNA oligo using NEBuilder HiFi DNA Assembly]( Optimization tips: [Optimization Tips for NEBuilder® HiFi DNA Assembly and NEB® Gibson Assembly]( --------------------------------------------------------------- Find out how Monarch® DNA & RNA purification kits are designed with sustainability in mind [Learn More]( --------------------------------------------------------------- New England Biolabs is a [Certified B Corporation®](. --------------------------------------------------------------- [New Products]( [Special Offers]( [Technical Support]( [Customer Feedback]( --------------------------------------------------------------- One or more of these products are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; rather, it indicates where the document was originally developed. For more information, please email busdev@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications. Your purchase, acceptance, and/or payment of and for NEB's products is pursuant to NEB's [terms of sale](. NEB does not agree to and is not bound by any other terms or conditions, unless those terms and conditions have been expressly agreed to in writing by a duly authorized officer of NEB. B Corporation® is a registered trademark of B Lab, Inc. Illumina® is a registered trademark of Illumina, Inc. Bio-Rad® is a registered trademark of Bio-Rad. ATCC® is a registered trademark of American Type Culture Collection Corporation. VR-3252SD™, VR-3229SD™ and VR-3246SD™ are trademarks of American Type Culture Collection Corporation. © Copyright 2022, New England Biolabs, Inc.; All rights reserved. New England Biolabs is an ISO 9001, ISO 14001 and ISO 13485 certified facility. NEB does not intend to inundate you with email messages. If you would like to update your email preferences or unsubscribe from the list, please [click here](. New England Biolabs, Inc. | 240 County Road | Ipswich, MA 01938-2723

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