Featured products for RT-qPCR, DNA assembly, DNA and RNA nucleic acid purification. APRIL 2022
Unable to view this email? [View online]( Itâs time to think differently. Many of you know NEB® as a trusted reagent supplier to the life science community, but did you know that we also offer a portfolio of products that can be used in infectious disease research, development of diagnostics and therapies, as well as in epidemiological studies and disease surveillance? [Many of our products have played a role in the global response to SARS-CoV-2]( from supporting the development of COVID-19 diagnostics and vaccines to [improving the speed and accuracy of Illumina® and Nanopore-based COVID-19 sample analysis](. They can also be utilized with other infectious diseases, such as influenza and malaria. Find out how NEB can support your infectious disease research and development. [Learn More](
--------------------------------------------------------------- Featured product:
Luna® Probe One-Step RT-qPCR 4X Mix with UDG (No ROX) Ideal for instruments that do not require the ROX passive reference dye, the Luna Probe One-Step RT-qPCR 4X Mix with UDG (No ROX) enables sensitive detection of target RNA sequences, with robust performance in multiplex applications of up to 5 targets. Detection of RNA viruses using the Luna Probe One-Step RT-qPCR 4X Mix with UDG (No ROX) RT-qPCR targeting SARS-CoV-2 (N1 target), Zika (POLY target), Dengue (E target), Chikungunya (E2 glycoprotein target) was performed using the Luna Probe One-Step RT-qPCR Mix with UDG (No ROX). Performance in 20 μl reactions was evaluated on a Bio-Rad® CFX96 real-time instrument over a 5-log range of template (100,000â10 copies/reaction). Templates including Synthetic SARS-CoV-2 RNA Control 2 (Twist Biosciences, SKU: 102024), Synthetic Zika virus (ZIKV) RNA (ATCC® VR-3252SDâ¢), Synthetic RNA from Dengue virus type 2 (ATCC VR-3229SDâ¢), Synthetic RNA from Chikungunya virus (ATCC VR-3246SDâ¢), were all individually diluted in 10 ng of Jurkat total RNA (BioChain, #R1255815-50). Sensitive, linear performance can be observed in the amplification of all viral targets. [Product Information](
Featured product:
NEBuilder® HiFi DNA Assembly Assembling DNA fragments is a key part of both synthetic biology techniques and cloning. [NEBuilder HiFi DNA Assembly]( enables virtually error-free joining of DNA fragments, even those with 5´- and 3´-end mismatches. This flexible kit enables simple and fast seamless cloning utilizing a proprietary high-fidelity polymerase, and offers improved performance over other commercially-available kits. Featured protocols: [Protocol for assembling annealed DNA oligonucleotides and a double-stranded DNA vector using NEBuilder HiFi DNA Assembly]( [Protocol for Bridging double-stranded DNA with a single-stranded DNA oligo using NEBuilder HiFi DNA Assembly](
Optimization tips: [Optimization Tips for NEBuilder® HiFi DNA Assembly and NEB® Gibson Assembly](
--------------------------------------------------------------- Find out how Monarch® DNA & RNA purification kits are designed with sustainability in mind [Learn More](
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