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Lyophilized LyoPrime Luna for RT-qPCR, Monarch HMW for DNA extraction, NEBridge Ligase MM for Golden Gate. FEBRUARY 2022 Unable to view this email? [View online]( New product: Introducing LyoPrime Luna™ Probe One-Step RT-qPCR Mix with UDG Supplied as a lyophilized cake, the LyoPrime Luna Probe One-Step RT-qPCR Mix with UDG features Luna® WarmStart® RT and enables sensitive detection of targeted RNA sequences. This is the first in a series of lyophilized products developed jointly by New England Biolabs and Fluorogenics™ Limited, which is now a subsidiary of New England Biolabs, Inc. - Simply add nuclease-free water for rapid rehydration - Store at room temperature for up to 2 years prior to rehydration - Eliminate cold chain shipping requirements - Multiplex up to 5 targets to increase throughput - Use with a variety of instrument platforms, including those that require a high or low ROX reference signal [Learn More About LyoPrime]( [Product Information]( Interested in learning more? Download our white paper: [Key considerations for optimal lyophilized reagent development](. Featured application: Isolating Ultra-High Molecular Weight (UHMW) DNA for Long Read Sequencing Applications The Monarch® HMW DNA Extraction Kits are designed for isolating UHMW/HMW gDNA for downstream nanopore ultra-long read DNA sequencing applications. Using a novel glass bead-based approach, these kits allow for extraction of DNA into the megabase (Mb) range quickly and easily, with best-in-class yields and purity. - Extract ultra-high molecular weight DNA into Mb range from cells and blood ([NEB #T3050]( or tissue, bacteria, and other sample types ([NEB #T3060]( - Use extracted DNA in Oxford Nanopore Technologies®, ultra-long & long read DNA sequencing applications - Generate high yields of highly pure DNA that is easily dissolvable and ready for sequencing - Save valuable time with fast workflows (30-90 minutes compared to days) - Generate DNA size and fragment length specific for your needs We’ve had great success with obtaining HMW DNA for long read sequencing from a variety of cell types, using less input and obtaining a comparable yield… It is straightforward and easy to use. – Inswasti Cahyani & Matt Loose, DeepSeq, University of Nottingham, UK [Learn More]( [Request a Sample]( --------------------------------------------------------------- Featured product: NEBridge® Ligase Master Mix Offering flexibility and convenience for users, NEBridge Ligase Master Mix is used for high efficiency and high-fidelity [Golden Gate Assembly]( with a broad assortment of [NEB Type IIS restriction enzymes](. 25-fragment Golden Gate Assembly of lac Cassette Using NEBridge Ligase Master Mix and Conventional T4 DNA Ligase Buffer A: 30 µl reactions containing 24 inserts and pGGAselect plasmid were set up using BbsI-HF ([NEB #R3539M](. Left plate shows results with 10 µl NEBridge Ligase Master Mix. Right plate shows results with 3 µl of 10X T4 DNA Ligase Buffer ([NEB #B0202]( and 1 µl of T4 DNA Ligase ([NEB #M0202T/M](. 1 µl of BbsI-HF was used for both reactions, as well as 0.05 pmol of each containing 24 insert DNAs and pGGAselect. Reactions proceeded for 60 cycles at 37°C for 5 min and 16°C for 5 min, with an end soak at 60°C for 5 min. 2 µl of each reaction was transformed in to 50 µl of T7 Express Competent E.coli ([NEB #C2566](. 50 µl of overgrowth was plated onto LB plates (supplemented with 1 mg/ml dextrose, 1 mg/ml MgCl2, 30 µg/ml chloramphenicol, 200 µM IPTG and 80 µg/ml X-gal) and incubated at 37°C for 18 hrs, and then stored at 4°C for 4 hrs before scoring colony color phenotype. B: The total transformants and percentage of correct assemblies (blue colonies) were reported as the average result of three replicates with the standard deviation from the mean. The reaction with NEBridge Ligase Master Mix generated 2.2 ±0.2X106 correctly assembled blue colonies per µg vector DNA with 94.3 ±1% fidelity, while the reaction with T4 DNA Ligase Buffer generated 8.3 ±2.1X105 correctly assembled blue colonies per µg vector DNA with 69.8 ± 10.7% fidelity. [Product Details]( [View Protocol]( --------------------------------------------------------------- New England Biolabs is a [Certified B Corporation®](. --------------------------------------------------------------- [New Products]( [Special Offers]( [Technical Support]( [Customer Feedback]( --------------------------------------------------------------- One or more of these products are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email busdev@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications. 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