Genomic DNA extraction, virus detection and golden gate assembly. JANUARY 2021
Unable to view this email? [View online]( Extracting Genomic DNA? Why not Migrate to Monarch®!
NEB offers two convenient solutions for the extraction of genomic DNA from biological samples.
--------------------------------------------------------------- Monarch HMW DNA Extraction Kits
Our novel, glass bead-based approach for the rapid isolation of ultra-high molecular weight DNA.
- Quickly and easily isolate High Molecular Weight (HMW) DNA from several sample types
- Tune the size of isolated DNA by adjusting the agitation speed during lysis
- Obtain best-in-class yields of highly pure and intact DNA for excellent performance in long read sequencing Missed our webinar? Watch it now.
The Product Developer of our Monarch HMW DNA Extraction kits introduces the technology, walks you through the workflow, and shares some long read sequencing data produced with purified HMW DNA.
[Learn More and
Request a Sample](
--------------------------------------------------------------- Monarch Genomic DNA Purification Kit
Our more traditional, spin-column-based workflow for gDNA extraction.
- Isolate large, intact gDNA fragments with a peak size >50 kb
- Purified DNA has outstanding quality metrics
- Perfect choice upstream of applications like cloning, qPCR and NGS This kit yielded the highest purity DNA I have ever seen from a commercial spin column kit. â Researcher, Lake Superior State University [Learn More and
Request a Sample]( Supporting COVID Research â Virus Detection
NEB® offers multiple products to identify the presence of SARS-CoV-2 viral RNA, based on RT-qPCR and loop-mediated isothermal amplification (LAMP). Featured research use only (RUO) products include: [Luna® SARS-CoV-2 RT-qPCR Multiplex Assay Kit]( for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes [Luna Probe One-Step RT-qPCR 4X Mix with UDG]( enables sensitive detection of target RNA sequences, with robust performance in multiplex applications with up to 5 targets. Learn about how the [Innovative Genomics Institute]( at University of California, Berkeley has designed a [novel SARS-CoV-2 assay]( using this product. [SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit]( for simple, visual detection (pink-to-yellow) of amplification of SARS-CoV-2 nucleic acid
--------------------------------------------------------------- Find out how NEB products are being used in COVID-19-related work, some of which has been approved as FDA EUA protocols. [Learn More](
--------------------------------------------------------------- Avoid domestication issues in your Golden Gate Assembly with PaqCI⢠[PaqCI]( is an isoschizomer of AarI. Unlike AarI, PaqCI cuts to completion and does not exhibit star activity under optimal conditions. PaqCI is a [Type IIS]( restriction enzyme that has a 7-basepair recognition sequence, useful for avoiding [domestication]( issues in [Golden Gate Assembly](. This enzyme requires [more than one site]( to efficiently cleave. To achieve optimal cleavage, PaqCI Activator is provided with the enzyme. We have provided a [protocol]( for Golden Gate Assembly using PaqCI, along with [guidelines]( for PaqCI Activator amounts. [Learn how]( you can push the limits of your Golden Gate Assembly, and find out how NEB has achieved high efficiency assembly with 35+ fragments. [Product Details](
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