A new solution for HMW DNA extraction – Request your trial kit

Faster workflows, improved yields and quality for cells, blood, tissue & bacteria Unable to view this email? [View online]( New products: Monarch® HMW DNA Extraction Kits NEB is pleased to announce the release of a powerful new solution for high molecular weight DNA extraction. The [Monarch HMW DNA Extraction Kits]( a novel glass bead-based approach, allowing users to extract DNA into the megabase (Mb) range quickly and easily, with best-in-class yields and purity from cells, blood, tissue and bacteria. Extracted HMW DNA is easy to dissolve and is often ready to use on the same day, significantly shortening the extraction process, which has long been a bottleneck for long read sequencing technologies. Highlights: - Fast workflows (cells: 30 minutes, blood: 60 minutes, tissue & bacteria: 90 minutes) - Extract DNA into the megabase (Mb) range from cells, blood, soft organ tissues, and bacteria - Tune DNA size based on agitation speed during lysis - Reproducibly achieve excellent yields and purity - Excellent performance in long read sequencing See how our Cells & Blood Kit works in [this short animation]( --------------------------------------------------------------- Workflow for extraction from cells --------------------------------------------------------------- Use of varying agitation speeds during lysis produces tunable fragment length of extracted HMW genomic DNA from cells and blood Preps were performed on duplicate aliquots of 1 x 106 HEK 293 cells and 500 µl fresh human blood. Samples were agitated at the indicated speed during the lysis step to control the fragmentation of the DNA. Equal amounts of DNA from the replicates (cells: 500 ng; blood: 650 ng) were resolved by PFGE (1% agarose gel, 6 V/cm, 13°C for 20 hours, switch times ramped from 0.5–94 seconds on a BioRad CHEF-DR III System). Yield and purity ratios of the individual preps are shown in the accompanying tables. Lambda PFG Ladder and Lambda DNA-Hind III Digest (NEB [#N0341]( and [#N3012]( were used as molecular weight standards. Yield, purity ratios and DINs of the individual preps are shown in the accompanying tables. --------------------------------------------------------------- We’ve had great success with obtaining HMW DNA for long read sequencing from a variety of cell types, using less input and obtaining a comparable yield… It is straightforward and easy to use. – Inswasti Cahyani & Matt Loose, DeepSeq, University of Nottingham --------------------------------------------------------------- Extracted DNA from HEK293, human blood and mouse kidney samples generates excellent sequencing results on the Oxford Nanopore Technologies® platform HEK293 Sample 1 HEK293 Sample 2 Human Blood Sample 1 Human Blood Sample 2 Mouse Kidney Sample Mean read length (bases) 21338.9 19249.9 21522.6 24677.7 27120.7 Mean read quality 12.8 13.2 13.4 13.3 13 Median read length (bases) 10388 9702 10130 12593 23150 Median read quality 13.2 13.7 13.9 13.8 13.5 Number of reads 377687 633636 538090 327314 164000 Read length N50 (bases) 45432 40415 46542 51394 44631 Total bases 8.1 Gb 12.2 Gb 11.6 Gb 8.1 Gb 4.4 Gb [Learn More and Request a Free Trial]( --------------------------------------------------------------- [New Products]( [Special Offers]( [Technical Support]( [Feedback]( [Upcoming Events]( --------------------------------------------------------------- One or more of these products are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please contact NEB’s Global Business Development team at [gbd@neb.com](mailto:gbd@neb.com?Subject=Patent,%20trademark%20and/or%20copyright%20information). The use of these products may require you to obtain additional third party intellectual property rights for certain applications. Your purchase, acceptance, and/or payment of and for NEB's products is pursuant to NEB's [terms of sale](. NEB does not agree to and is not bound by any other terms or conditions, unless those terms and conditions have been expressly agreed to in writing by a duly authorized officer of NEB. Oxford Nanopore Technologies® is a registered trademark of Oxford Nanopore Technologies Ltd. © Copyright 2020, New England Biolabs, Inc.; All rights reserved. New England Biolabs is an ISO 9001, ISO 14001 and ISO 13485 certified facility. NEB does not intend to inundate you with email messages. If you would like to update your email preferences or unsubscribe from the list, please [click here](. New England Biolabs, Inc. | 240 County Road | Ipswich, MA 01938-2723