New products and protocols to support COVID-19 research

Detection of target RNA sequences and extraction of viral RNA from saliva, buccal swabs and a simulated respiratory secretions NOVEMBER 2020 Unable to view this email? [View online]( Your science is essential, and we thank you for your commitment to your research, whether you are studying SARS-CoV-2 or other important topics. At NEB, we continue to supply and support researchers around the world who are working to develop diagnostics and vaccines for the SARS-CoV-2 virus. Our team is also monitoring the literature and collecting citations and EUA protocols enabled by NEB reagents, so you will always have an [up-to-date resource](. [View NEB’s latest preprint]( which outlines a multiplex LAMP assay that can distinguish SARS-CoV-2 from Influenza A and B viral RNA in a single reaction. --------------------------------------------------------------- New product: Luna® Probe One-Step RT-qPCR 4X Mix with UDG The [Luna Probe One-Step RT-qPCR 4X Mix with UDG]( enables sensitive detection of target RNA sequences, with robust performance in multiplex applications of up to 5 targets. - Simplify your reaction setup with a single-tube master mix format - Increase sensitivity of your RT-qPCR, as 4X concentration allows for more sample input - Luna WarmStart® RT paired with Hot Start Taq increases reaction specificity and robustness, allowing for room temperature setup - Room temperature stability up to 24 hours (no spurious amplification or loss of sensitivity) - Reduce risk of carryover contamination, with UDG and dUTP included in the mix - Eliminate pipetting errors with non-interfering, visible blue tracking dye The Luna Probe One-Step RT-qPCR 4X Mix with UDG outperforms comparable products One-step RT-qPCR was tested on 8 RT-qPCR targets (indicated by color) varying in abundance, length, and %GC. Data was collected on multiple days by two users according to manufacturer’s recommendations using the Applied Biosystems™ QuantStudio™ 6 real-time PCR system. Results were evaluated for efficiency, low input detection and lack of non-template amplification (where ΔCq = average Cq of non-template control – average Cq of lowest input). In addition, consistency, reproducibility and overall curve quality were assessed based on metrics described previously (Quality Score). Although both products performed reasonably well, NEB’s Luna Probe RT-qPCR 4X Mix with UDG outperformed the TaqPath 1-Step RT-qPCR Master Mix, CG, as evidenced by the higher number of experiments whose results fell in the green box. For more information on the “dots in boxes” data visualization method, please visit [www.neb.com/M3019](. [Learn More]( --------------------------------------------------------------- Featured technical note: Expanding the utility of Monarch® RNA purification kits to support COVID-19 research The [Monarch Total RNA Miniprep Kit]( and [Monarch RNA Cleanup Kits]( can successfully purify synthetic SARS-CoV-2 viral RNA from clinically-relevant samples. - Linear, quantitative recovery of the SARS-CoV-2 N-gene from purified RNA over a 5-log range (50 to 500,000 copies) confirmed using RT-qPCR - New, streamlined Monarch Total RNA Miniprep Kit saliva protocol shortens sample processing time by ~30 minutes - With the addition of [DNA/RNA Protection Reagent]( Monarch RNA Cleanup Kits enable fast extraction of viral RNA from saliva, buccal swabs and a simulated respiratory secretion - Workflows can be automated on the QIAcube® and KingFisher® Flex platforms The Monarch Total RNA Miniprep Kit successfully purifies synthetic SARS-CoV-2 viral RNA from saliva samples The Monarch Total RNA Miniprep Kit Proteinase K and Quick Protocols were used to isolate total RNA from saliva samples containing 10-fold serial dilutions of synthetic SARS-CoV-2 N-gene RNA. Purified RNA was eluted in 100 µl nuclease-free water to yield 50 to 500,000 copies of viral RNA/µl. Using the Luna Universal Probe One-Step RT-qPCR Kit (NEB #E3006), titers as low as 50 copies (the lowest input tested) were detected and linear, quantitative recovery of the SARS-CoV-2 N-gene was observed over a 5-Log range. [Download Our Technical Note]( [Visit Our COVID Page to Access Protocols]( --------------------------------------------------------------- [New Products]( [Special Offers]( [Technical Support]( [Feedback]( [Upcoming Events]( --------------------------------------------------------------- One or more of these products are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please contact NEB’s Global Business Development team at [gbd@neb.com](mailto:gbd@neb.com?Subject=Patent,%20trademark%20and/or%20copyright%20information). The use of these products may require you to obtain additional third party intellectual property rights for certain applications. Your purchase, acceptance, and/or payment of and for NEB's products is pursuant to NEB's [terms of sale](. NEB does not agree to and is not bound by any other terms or conditions, unless those terms and conditions have been expressly agreed to in writing by a duly authorized officer of NEB. TAQPATH™ is a trademark of Thermo Fisher Scientific. APPLIED BIOSYSTEMS™ and QUANTSTUDIO™ are trademarks of Applied Biosystems, Pty, Ltd. QIACUBE® is a registered trademark of Qiagen GmbH. KINGFISHER® is a registered trademark of Thermo Fisher Scientific. © Copyright 2020, New England Biolabs, Inc.; All rights reserved. New England Biolabs is an ISO 9001, ISO 14001 and ISO 13485 certified facility. NEB does not intend to inundate you with email messages. If you would like to update your email preferences or unsubscribe from the list, please [click here](. New England Biolabs, Inc. | 240 County Road | Ipswich, MA 01938-2723