Is this email not displaying correctly? [View it in your browser](. Dear {NAME}, Join us for our two-part webinar: Abundant transcripts in your sample?
Where they come from and how to focus on sequences that matter to you The large dynamic range of transcript expression presents a challenge in whole-transcriptome sequencing. Highly expressed transcripts, their sources, and how they can hinder detection of informative transcripts will be discussed. We have optimized a method to enrich for transcripts of interest by eliminating specific RNAs before sequencing. This method is based on the hybridization of probes to unwanted RNAs followed by enzymatic degradation of the targeted RNAs and probes. In this webinar we present our latest solutions, including a customization option, to enrich for RNAs of interest across diverse species. In part 1, NEB scientists Deyra Rodriguez and Bradley Langhorst will present advances in our method to eliminate cytoplasmic and mitochondrial ribosomal RNA (rRNA) from human, mouse, and rat total RNA samples. This method has been successfully applied to a range of total RNA samples including from formalin-fixed-paraffin-embedded (FFPE). Additionally, they will present a solution to remove abundant adult, fetal and embryonic globin transcripts from blood RNA samples in human, mouse and rat. In part 2, Brad, Deyra and Keerthana Krishnan will discuss a solution for removal of rRNA from a wide variety of bacterial species. Furthermore, they will introduce a new customizable approach to remove any RNA of interest from your favorite sample, and demonstrate a user-friendly, easily accessible web tool to enable custom probe design. Probes can be designed to target RNAs in single species, multiple species, or used to supplement existing probe sets. After the tool demonstration, they will provide guidelines for evaluating your custom design. [Register Here]( PART I:
Depletion of ribosomal and globin RNAs from human, mouse and rat samples
Date: Thursday, November 5th
Time: 2:00 PM EDT PART II:
Ribosomal RNA removal from bacterial samples, and customized RNA depletion from any species
Date: Tuesday, November 10th
Time: 2:00 PM EDT Deyra N. RodrÃguez, Ph.D., M.B.A. is an Applications and Product Development Scientist in the NEBNext® team at New England Biolabs (NEB). Since joining NEB in 2013, Dr. RodrÃguez has focused on the co-development of products for RNA-seq applications including small RNA, whole transcriptome, and the expansion of the RNA depletion product line (rRNA, globin and custom). She received her Ph.D. in Genetics from the Stanford University School of Medicine and her M.B.A from Rochester Institute of Technology. Dr. Bradley W. Langhorst is a Development Group Leader at New England Biolabs (NEB). He has been working with high-throughput sequence data since his time at the Whitehead Institute Center for Genome Research in the late 90s. Brad earned a Ph.D. in Biochemistry from the University of New Hampshire under the supervision of Tom Laue, before joining New England Biolabs as a Postdoc in 2008. He finds satisfaction in constructing useful tools and methods to empower researchers and product developers both within NEB and in the broader community. Dr. Keerthana Krishnan is an Applications and Product Development Scientist within the NEBNext team at New England Biolabs (NEB). She received her Ph.D. in Cancer Genomics from the University of Queensland (Australia) under the mentorship of Prof. Sean Grimmond. She then managed the sequencing core at The Forsyth Institute (Cambridge, MA), overseeing projects spanning different applications including 16S, WGS and RNA-Seq. She joined New England Biolabs in 2015, where her focus has been co-developing products for several RNA-Seq applications including whole transcriptome, single cell, bacterial depletion and adaptors for RNA library preparation.
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